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未来化学科技有限公司
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发布时间：
2005-12-09
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资料介绍
本文介绍了使用超临界CO2萃取方法萃取微藻中类胡罗卜素和藻青蛋白等生物活性物质,详细全文请到未来化学科技有限公司网站下载:http://www.futurechemtech.com
Extraction of Astaxantine and Phycocyanine from
Microalgae with Supercritical Carbon Dioxide -----
Astaxantine from the microalga Haematococcus pluvialis and phycocyanine from the microalga Spirulina
maxima have been obtained by supercritical extraction using carbon dioxide. Prior to extraction, the
samples of microalgae were crushed by cutting mills (coffee mill) and then manually ground with dry ice
(solid carbon dioxide). The Haematococcus extracts were analyzed by liquid chromatography, using
astaxantin (purity of 98%) as a standard. Phycocyanine, being insoluble in carbon dioxide, was indirectly
separated. Thus, lipid-soluble substances from spirulina were extracted and analyzed by liquid
chromatography, using as a standard a solution prepared on the base of reagent (purity of 40%). For the
astaxantine extraction, the results show that cellular wall breaking has an important effect on extraction
efficiency. The addition of a cosolvent (9.4 mass % of ethanol) has little effect, reaching an extraction
yield of about 1.7 mass %. The extraction yield is much below the expected lipid content in dry
Haematococcus alga samples, which is known to be close to 30 mass %. The maximum total recovery of
astaxantin, calculated from its initial and residual content in the alga (0.0147 and 0.0004, respectively),
exceeds 97%. For phycocyanine extraction, the addition of cosolvent (10 mass % of ethanol) has a strong
effect on the extraction yield of lipidic substances. This value goes from about 1.1% for extraction without
cosolvent to about 1.7 mass % when the cosolvent is used. The total extraction yield of about 3 mass %
corresponds well to the average lipid content of 3.27% in alga Spirulina maxima reported in the literature.Astaxantine from the microalga Haematococcus pluvialis and phycocyanine from the microalga Spirulina
maxima have been obtained by supercritical extraction using carbon dioxide. Prior to extraction, the
samples of microalgae were crushed by cutting mills (coffee mill) and then manually ground with dry ice
(solid carbon dioxide). The Haematococcus extracts were analyzed by liquid chromatography, using
astaxantin (purity of 98%) as a standard. Phycocyanine, being insoluble in carbon dioxide, was indirectly
separated. Thus, lipid-soluble substances from spirulina were extracted and analyzed by liquid
chromatography, using as a standard a solution prepared on the base of reagent (purity of 40%). For the
astaxantine extraction, the results show that cellular wall breaking has an important effect on extraction
efficiency. The addition of a cosolvent (9.4 mass % of ethanol) has little effect, reaching an extraction
yield of about 1.7 mass %. The extraction yield is much below the expected lipid content in dry
Haematococcus alga samples, which is known to be close to 30 mass %. The maximum total recovery of
astaxantin, calculated from its initial and residual content in the alga (0.0147 and 0.0004, respectively),
exceeds 97%. For phycocyanine extraction, the addition of cosolvent (10 mass % of ethanol) has a strong
effect on the extraction yield of lipidic substances. This value goes from about 1.1% for extraction without
cosolvent to about 1.7 mass % when the cosolvent is used. The total extraction yield of about 3 mass %
corresponds well to the average lipid content of 3.27% in alga Spirulina maxima reported in the literature.
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